- The 17th-century church of Ayia Irini near the port's mouth.  
- Homer's grave at Plakoto.Ruins of an ancient aqueduct at Plakoto. 
- The church of Ai Georgis at Yialos. 
- Panayia Gremiotissa in Hora; the church is a fine example of Byzantine architecture and is dedicated to the Dormition of the Virgin.  
- Panayia Katholiki. 
- The church of Evangelismos (Assumption), the island's cathedral.  
- Ruins of the Byzantine castle at Paliokastro. 
- Psathopyrgos at Plakoto. 
- Ayios Ioannis at Kalamos. 
- Ayios Ioannis at Pyrgos; the church is built over the ruins of a temple dedicated to Apollo. 
- The Anemomyloi quarter with its 12 windmills. 
- The modern 1,000-seat open-air marble Odysseus Elytis Theater.  
- The Folk Museum 
- The Ios Archaeological Museum 
- The Modern Art Museum at Kolitsanous.